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pAP114
(Plasmid #120799)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 120799 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDSW1728
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 8513
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Low copy in C. difficile following conjugation and selected with thiamphenicol
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pxyl::mCherryOpt
  • Species
    C. difficile
  • Insert Size (bp)
    2093
  • Mutation
    1.4 kb xylR-Pxyl DNA fragment from C. difficile R20291 controls expression of mCherryOpt
  • Promoter Pxyl

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAP114 was a gift from Craig Ellermeier & David S. Weiss (Addgene plasmid # 120799 ; http://n2t.net/addgene:120799 ; RRID:Addgene_120799)
  • For your References section:

    A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile. Muh U, Pannullo AG, Weiss DS, Ellermeier CD. J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18. 10.1128/JB.00711-18 PubMed 30745377