Functionally diverse type V CRISPR-Cas systems.
Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng DR, Scott DA
Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. Epub 2018 Dec 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120872 | pET28a-mH6-Cas12c1 | Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone. |
| 120873 | pET28a-mH6-Cas12c2 | Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone. |
| 120877 | pACYC-Cas12c2-noncoding | Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity. |
| 120878 | pACYC-OspCas12c-noncoding | Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity. |
| 120879 | pET28a-mH6-Cas12g1 | Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone. |
| 120880 | pACYC-Cas12g1-noncoding | Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity. |
| 120882 | pET28a-mH6-Cas12i1 | Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone. |
| 120883 | pET28a-mH6-Cas12i2 | Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone. |