PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120883||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5838
- Total vector size (bp) 9423
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)3168
- Promoter lac
/ Fusion Protein
- mH6 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-mH6-Cas12i2 was a gift from Arbor Biotechnologies (Addgene plasmid # 120883 ; http://n2t.net/addgene:120883 ; RRID:Addgene_120883)
For your References section:Functionally diverse type V CRISPR-Cas systems. Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng DR, Scott DA. Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. Epub 2018 Dec 6. 10.1126/science.aav7271 PubMed 30523077