Two-step pathway for isoprenoid synthesis.

Chatzivasileiou AO, Ward V, Edgar SM, Stephanopoulos G
Proc Natl Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 10.1073/pnas.1812935116. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
122017	p5T7-LYCipi-ggpps	Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.
122018	pSEVA228-pro4IUPi	Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.
122019	pTET-IUPi	Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.