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Multiplexed orthogonal genome editing and transcriptional activation by Cas12a.
Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler L, Cobos Galvez A, Tschaharganeh DF
Nat Methods. 2019 Jan;16(1):51-54. doi: 10.1038/s41592-018-0262-1. Epub 2018 Dec 17. PubMed

Plasmids from Article

ID Plasmid Purpose
122244pMB22Lentivirus delivery for stable expression of AsCas12a-VPR
122245pMB34Lentivirus delivery for stable expression of LbCas12a-VPR
122246pMB4Lentivirus delivery for stable expression of As crRNA, has AsDR; Cloning vector for expression of AsCpf1 crRNA. It contains BsmBI site for cloning.
122247pMB30Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

Antibodies from Article