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Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents.
Cho YK, Park D, Yang A, Chen F, Chuong AS, Klapoetke NC, Boyden ES
J Biol Chem. 2019 Jan 4. pii: RA118.006996. doi: 10.1074/jbc.RA118.006996.
PubMed Article

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ID Plasmid Purpose  
123125pAAV-GFAP104-ChromeQ-mCherryAAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.
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123126pAAV-GFAP104-ChromeT-mCherryAAV-mediated expression of ChromeT-mCherry under the GFAP promoter.
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123316pAAV-Syn-ChromeT-GFPAAV-mediated expression of ChromeT-GFP under the Syn promoter.
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123317pAAV-Syn-ChromeQ-GFPAAV-mediated expression of ChromeQ-GFP under the Syn promoter.
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153539pAAV-Syn-FLEX-rc [ChromeT-GFP]AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.
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153540pAAV-Syn-FLEX-rc [ChromeQ-GFP]AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.
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