PurposeAAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123125||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4915
- Total vector size (bp) 6571
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsCan use DH5alpha at 37°C or Stbl3 at 30°C. Carbenicillin is preferred over ampicillin. In DH5alpha this plasmid may act more like a high copy plasmid, although in Stbl3 it may act more like a low copy plasmid.
Copy numberHigh Copy
Insert Size (bp)1656
MutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E90A/H114G/R115S
- Promoter GFAP104
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ggagagctctccccatag
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-GFAP104-ChromeQ-mCherry was a gift from Edward Boyden (Addgene plasmid # 123125 ; http://n2t.net/addgene:123125 ; RRID:Addgene_123125)
For your References section:Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents. Cho YK, Park D, Yang A, Chen F, Chuong AS, Klapoetke NC, Boyden ES. J Biol Chem. 2019 Jan 4. pii: RA118.006996. doi: 10.1074/jbc.RA118.006996. 10.1074/jbc.RA118.006996 PubMed 30610117