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Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Grunewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123611nCas9-UGI-NLS-P2A-EGFP (pJUL1001)CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).
123612BE3-P2A-EGFP (pJUL977)CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.
123613BE3(E63Q)-P2A-EGFP (pRZ189)CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).
123614SECURE BE3(R33A)-P2A-EGFP (pJUL1085)CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).
123615SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
123616NLS-nCas9-NLS-P2A-EGFP (pRZ70)CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).