Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor.

Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S
Science. 2019 Mar 8;363(6431):1103-1107. doi: 10.1126/science.aaw2859. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
123618	pDDGFP-Leu2d-GgKDELR2	Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123619	pDDGFP-Leu2d-GgKDELR2 H12A	Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123620	pDDGFP-Leu2d-GgKDELR2 R47A	Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123621	pDDGFP-Leu2d-GgKDELR2 R47K	Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123622	pDDGFP-Leu2d-GgKDELR2 E127A	Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123623	pDDGFP-Leu2d-GgKDELR2 E127Q	Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123624	pDDGFP-Leu2d-GgKDELR2 Y158F	Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123625	pDDGFP-Leu2d-GgKDELR2 R159A	Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123626	pDDGFP-Leu2d-GgKDELR2 R159K	Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection
123627	pBXPC3H-Syb37	Expresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection