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A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs.
Talas A, Huszar K, Kulcsar PI, Varga JK, Varga E, Toth E, Welker Z, Erdos G, Pach PF, Welker A, Gyorgypal Z, Tusnady GE, Welker E
Nucleic Acids Res. 2021 Jan 15. pii: 6101605. doi: 10.1093/nar/gkaa1220.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
124221pAT-9208Cloning plasmid for a self-targeting gRNA library
124222pAT-9222Cloning plasmid for EGxFP assay with self-targeting gRNA-s
124225pAT-9218Bacterial SpCas9 expression
124226pAT-9251Bacterial SpCas9-HF1 expression
124227pKH-1699Mammalian SpCas9-HF1 expression