PurposeBacterial SpCas9 expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||124225||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberLow Copy
- Promoter TetR/TetA
- Cloning method Gibson Cloning
- 5′ sequencing primer CGACCTCATTAAGCAGCTCT
- 3′ sequencing primer GAGGAAGCGGAATATATCCCTAG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAT-9218 was a gift from Ervin Welker (Addgene plasmid # 124225 ; http://n2t.net/addgene:124225 ; RRID:Addgene_124225)
For your References section:A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs. Talas A, Huszar K, Kulcsar PI, Varga JK, Varga E, Toth E, Welker Z, Erdos G, Pach PF, Welker A, Gyorgypal Z, Tusnady GE, Welker E. Nucleic Acids Res. 2021 Jan 15. pii: 6101605. doi: 10.1093/nar/gkaa1220. 10.1093/nar/gkaa1220 PubMed 33450024