Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II.
Nagashima R, Hibino K, Ashwin SS, Babokhov M, Fujishiro S, Imai R, Nozaki T, Tamura S, Tani T, Kimura H, Shribak M, Kanemaki MT, Sasai M, Maeshima K
J Cell Biol. 2019 Mar 1. pii: jcb.201811090. doi: 10.1083/jcb.201811090.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124495 | POLR2A-N CRISPR pX330 | A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A |
| 124496 | mAC-POLR2A donor (Hygro) | A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover |
| 247344 | pEF-1α-Venus-Nup107-FRT | Expresses Venus-NUP107 under EF1 promoter and can be integrated into FRT site |
| 247345 | pPB-CAG-IB-H2B-HaloTag | Expresses H2B-Halo under CAG promoter and can be randomly integrated by PiggyBac transposase |