Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing.
Chaverra-Rodriguez D, Macias VM, Hughes GL, Pujhari S, Suzuki Y, Peterson DR, Kim D, McKeand S, Rasgon JL
Nat Commun. 2018 Aug 1;9(1):3008. doi: 10.1038/s41467-018-05425-9.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
242758 | pET28(a)-p2c-eGFP | Expresses eGFP fluorophore with fused p2c targeting ligand for expression in E. coli |
242759 | pET28(a)-p2c-Cas9 | Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli |