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Modulating the Expression Strength of the Baculovirus/Insect Cell Expression System: A Toolbox Applied to the Human Tumor Suppressor SMARCB1/SNF5.

Golas MM, Jayaprakash S, Le LTM, Zhao Z, Heras Huertas V, Jensen IS, Yuan J, Sander B
Mol Biotechnol. 2018 Nov;60(11):820-832. doi: 10.1007/s12033-018-0107-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
109174pGS-BacA-21122Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems
109175pGS-BacA-21222Plasmid for expression of C-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems
109177pGS-BacA-21232Plasmid for expression of C-terminally CBP-3xFLAG-ProteinA-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems
109180pGS-BacA-23012Plasmid for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems
109181pGS-BacA-23122Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems
109183pGS-BacD-23003Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems
109184pGS-BacD-23004Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems
109185pGS-BacD-23005Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems
109186pGS-BacA-21222-SNF5Plasmid for expression of C-terminally 3xFLAG-tagged SNF5 under the control of a polh promoter in baculovirus/insect cell systems

Antibodies from Article