Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization.

Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
127396	pFUGW Cas9-BFP	Cas9 expression
127397	library backbone 3'LTR gRNA and barcode	Digestion by BstXI and BamHI to release the backbone