Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids.
Bosch JA, Colbeth R, Zirin J, Perrimon N
Genetics. 2019 Nov 4. pii: genetics.119.302819. doi: 10.1534/genetics.119.302819.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127553 | pCFD3-frame_selector_0 | Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in |
| 127554 | pCFD3-frame_selector_1 | Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in |
| 127555 | pCFD3-frame_selector_2 | Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in |
| 127556 | pCRISPaint-T2A-Gal4-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker. |
| 127557 | pCRISPaint-T2A-sfGFP-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker. |
| 127558 | pCRISPaint-T2A-LexGAD-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker. |
| 127559 | pCRISPaint-T2A-QF2-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker. |
| 127560 | pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker. |
| 127561 | pCRISPaint-T2A-FLPo-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker. |
| 127562 | pCRISPaint-T2A-Gal80-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker. |
| 127563 | pCRISPaint-T2A-Nluc-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker. |
| 127564 | pCRISPaint-T2A-Gal4DBD-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker. |
| 127565 | pCRISPaint-T2A-p65-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker. |
| 127566 | pCRISPaint-sfGFP-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker. |
| 130276 | pCRISPaint-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site. |
| 130277 | pCRISPaint-3xP3-GFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site. |
| 130278 | pCFD3-Act5c | Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells. |
| 131152 | pCFD5-frame_selector_0,1,2 | Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in |