Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids.
Bosch JA, Colbeth R, Zirin J, Perrimon N
Genetics. 2019 Nov 4. pii: genetics.119.302819. doi: 10.1534/genetics.119.302819. PubMed Article
Bosch JA, Colbeth R, Zirin J, Perrimon N
Genetics. 2019 Nov 4. pii: genetics.119.302819. doi: 10.1534/genetics.119.302819. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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127553 | pCFD3-frame_selector_0 | Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in |
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127554 | pCFD3-frame_selector_1 | Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in |
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127555 | pCFD3-frame_selector_2 | Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in |
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127556 | pCRISPaint-T2A-Gal4-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker. |
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127557 | pCRISPaint-T2A-sfGFP-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker. |
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127558 | pCRISPaint-T2A-LexGAD-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker. |
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127559 | pCRISPaint-T2A-QF2-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker. |
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127560 | pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker. |
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127561 | pCRISPaint-T2A-FLPo-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker. |
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127562 | pCRISPaint-T2A-Gal80-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker. |
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127563 | pCRISPaint-T2A-Nluc-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker. |
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127564 | pCRISPaint-T2A-Gal4DBD-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker. |
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127565 | pCRISPaint-T2A-p65-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker. |
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127566 | pCRISPaint-sfGFP-3xP3-RFP | CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker. |
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131152 | pCFD5-frame_selector_0,1,2 | Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in |
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