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LADL: light-activated dynamic looping for endogenous gene expression control.
Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE
Nat Methods. 2019 Jun 24. pii: 10.1038/s41592-019-0436-5. doi: 10.1038/s41592-019-0436-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127664LADL AnchorEncodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter
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127665Empty Anchor ControlEncodes for puromycin resistance expressed from the EF1a promoter
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127666Empty Bridge Control Zfp462-Klf4SEEncodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.
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127667Empty Target ControlEncodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter
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127668LADL Bridge + Target Zfp462-Klf4SEEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
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127669LADL Bridge + Promoter Only TargetEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters
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127670S12.1_Empty_BridgeAmpicillin resistant multiplexing backbone without CRY2HA and mCherry
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127671S12.2_Bridge + TargetAmpicillin resistant multiplexing backbone with CRY2HA and mCherry
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