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Microhomology based CRISPR tagging tools for protein tracking, purification, and depletion.
Lin DW, Chung BP, Huang JW, Wang X, Huang L, Kaiser P
J Biol Chem. 2019 May 28. pii: RA119.008422. doi: 10.1074/jbc.RA119.008422.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127875pX330-BbsI-PITChFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly
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127881pN-PITCh-3XHAProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging
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127882pN-PITCh-HFProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging
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127883pN-PITCh-HBTHProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging
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127884pN-PITCh-H3FAIDProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging
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127885pN-PITCh-FAIDProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging
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127887pN-PITCh-mCherryProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging
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127888pN-PITCh-GFPProvides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging
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