About Search by Sequence
-
Search by Sequence performs a nucleotide-nucleotide BLAST search against Addgene’s plasmid sequence database.
BLAST returns plasmids with similarity to the query sequence.
-
Results are sorted by E-value, a statistic from BLAST that describes the significance of a match.
Lower values are considered better matches.
-
FASTA headers and numbers at the beginning of each line will be removed.
The query should only contain DNA characters.
-
The minimum query length is 30 nucleotides. Support for short sequence queries is under development.
Tips for Success
-
Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory.
Visit the corresponding plasmid webpage to view additional details about a matching plasmid.
-
If no results are returned, try a different isoform or region of the desired sequence. There may not be a match in our database.
-
You can adjust the Max Results setting on the results page from 25 to 500. If many sequences share the same top E-value,
only a truncated set of equally high-scoring matches will be shown. Set the Max Results to 500 to see more matches.
-
Fill out our Suggest a Plasmid form or
e-mail [email protected] to let us know what we're missing.
By using search by sequence, you understand that some sequences are subject to our Terms of Access and the restrictions contained therein.