Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria.
Liu Y, Wan X, Wang B
Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0. PubMed Article
Liu Y, Wan X, Wang B
Nat Commun. 2019 Aug 26;10(1):3693. doi: 10.1038/s41467-019-11479-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130907 | pLY9 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV). |
| 130911 | pLY53 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV). |
| 130923 | pLY54 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV). |
| 130924 | pLY55 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV). |
| 130925 | pLY56 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV). |
| 130926 | pLY57 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV). |
| 130927 | pLY59 | A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV). |
| 130928 | pLY61 | A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV). |
| 130937 | pLY103 | Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130938 | pLY108 | Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130939 | pLY105 | Engineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130940 | pLY110 | Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130941 | pLY106 | Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130942 | pLY111 | Engineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130943 | pLY107 | Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130944 | pLY112 | Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130945 | pLY74 | Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130946 | pLY124 | Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad |
| 130947 | pLY151 | pspFΔHTH::λN22plus gene controlled by promoter PrhaB |
| 130948 | pLY70 | A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV). |
| 130949 | pLY86 | Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold |
| 130950 | pLY152 | dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV) |
| 130951 | pLY153 | reporter circuit (PpspA-2G6 with sfgfp::ASV) |
| 130952 | pLY130 | Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly |
| 130956 | pLY131 | Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly |
| 130960 | pLY132 | Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly |
| 130961 | pLY133 | Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly |
| 130962 | pLY134 | Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly |
| 130963 | pLY76 | Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2 |
| 130964 | pLY77 | Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2 |