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Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria
Yang Liu, Xinyi Wan & Baojun Wang
Nature Communications 10, August 2019
Article

Plasmids from Article

ID Plasmid Purpose  
130907pLY9A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
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130911pLY53A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
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130923pLY54A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
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130924pLY55A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
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130925pLY56A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).
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130926pLY57A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).
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130927pLY59A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).
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130928pLY61A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
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130937pLY103Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2
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130938pLY108Engineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2
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130939pLY105Engineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2
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130940pLY110Engineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2
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130941pLY106Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2
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130942pLY111Engineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2
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130943pLY107Engineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2
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130944pLY112Engineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2
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130945pLY74Engineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2
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130946pLY124Engineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter Pbad
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130947pLY151pspFΔHTH::λN22plus gene controlled by promoter PrhaB
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130948pLY70A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
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130949pLY86Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold
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130950pLY152dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
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130951pLY153reporter circuit (PpspA-2G6 with sfgfp::ASV)
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130952pLY130Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly
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130956pLY131Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly
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130960pLY132Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly
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130961pLY133Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly
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130962pLY134Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
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130963pLY76Engineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2
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130964pLY77Engineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2
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