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Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing.
Acharya S, Mishra A, Paul D, Ansari AH, Azhar M, Kumar M, Rauthan R, Sharma N, Aich M, Sinha D, Sharma S, Jain S, Ray A, Jain S, Ramalingam S, Maiti S, Chakraborty D
Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
130966pET-His6-FnCas9GFPexpression of FnCas9-GFP in bacterial cells
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130968PX458-3xHA-SpCas93xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA
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130969PX458-3xHA-FnCas93xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA
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