In vivo monitoring of plant small GTPase activation using a Forster resonance energy transfer biosensor.
Wong HL, Akamatsu A, Wang Q, Higuchi M, Matsuda T, Okuda J, Kosami KI, Inada N, Kawasaki T, Kaneko-Kawano T, Nagawa S, Tan L, Kawano Y, Shimamoto K
Plant Methods. 2018 Jul 7;14:56. doi: 10.1186/s13007-018-0325-4. eCollection 2018. PubMed Article
Wong HL, Akamatsu A, Wang Q, Higuchi M, Matsuda T, Okuda J, Kosami KI, Inada N, Kawasaki T, Kaneko-Kawano T, Nagawa S, Tan L, Kawano Y, Shimamoto K
Plant Methods. 2018 Jul 7;14:56. doi: 10.1186/s13007-018-0325-4. eCollection 2018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133262 | Raichu-OsRac1 WT | Biosensor for monitoring activation for rice small GTPase OsRac1 |
| 133263 | Raichu-OsRac1 DN | Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays |
| 133264 | Raichu-OsRac1 CA | Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays. |