Raichu-OsRac1 CA
(Plasmid #133264)

• Purpose: Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.
• Depositing Lab: Yoji Kawano
• Publication: Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10.1186/s13007-018-0325-4. eCollection 2018.

Backbone

• Vector backbone: pBI221
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsRac1(G19V)
• Species: Oryza sativa
• Mutation: OsRac1 G19V
• Entrez Gene: LOC4325879 (a.k.a. OSNPB_010229400, OsRac1, Rac1)
• Promoter: Ubi
• Tags / Fusion Proteins:
  • Venus-CRIB (N terminal on insert)
  • CFP-lipid (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pBI221 35S: ACTGACGTAAGGGATGACGC
• 3′ sequencing primer: NosTer R 2: GATAATCATCGCAAGACCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry