K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site.
Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark KA, Minor DL Jr
Nature. 2017 Jul 20;547(7663):364-368. doi: 10.1038/nature22988. Epub 2017 Jul 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133269 | pPICZ:mTREK-1_cryst | P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP |
| 133270 | pGEMHE:mTREK-1(K271Q) | X. laevis expression vector. It will generate the mouse TREK-1 channel |
| 133271 | pGEMHE:mTREK-1_cryst | X. laevis expression vector. It will generate the TREK-1 channel |
| 133272 | pGEMHE:mTREK-1(G137I) | X. laevis expression vector. It will generate the mouse TREK-1 channel |
| 133273 | pIRES:mTREK-1(K271Q) | Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag |
| 133274 | pIRES:mTREK-1(G137I) | Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag |