Altered expression of a quality control protease in E. coli reshapes the in vivo mutational landscape of a model enzyme.

Thompson S, Zhang Y, Ingle C, Reynolds KA, Kortemme T
eLife. 2020 Jul 23;9. pii: 53476. doi: 10.7554/eLife.53476. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
134815	SMT101	Dual expression of DHFR and TYMS, in vivo assays
134816	SMT201	SMT101 with TET promoter for TYMS, in vivo assays
134817	SMT205	SMT201 with mutated RBS for DHFR, in vivo assays
134818	SMT215	SMT205 with DHFR-FLAG-tag, western blot