Expression of Ca(2)(+)-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells.
Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, Parrington J, Galione A
EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. Epub 2015 Apr 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135182 | TPC1-mCherry | Expresses TPC1 with C-terminus mCherry tag in mammalian cells |
| 135183 | TPC2-mCherry | Expresses TPC2 with C-terminus mCherry tag in mammalian cells |
| 135184 | TPC1(ΔN69)-mCherry | TPC1 with truncated N-terminus, tagged with mCherry on C terminus. |
| 135185 | TPC2(ΔN49)-mCherry | TPC2 with truncated N-terminus, tagged with mCherry on C terminus. |
| 135186 | TPC2(N257A)-mCherry | Pore-dead mutant of TPC2, tagged on C terminus with mCherry. |
| 135187 | TPC2(E643A)-mCherry | Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry. |
| 135189 | TRPML1-mCherry | Expresses TRPML1 with C-terminus mCherry tag in mammalian cells. |