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A high-throughput screening method to identify proteins involved in unfolded protein response of the endoplasmic reticulum in plants.
Alcantara A, Seitner D, Navarrete F, Djamei A
Plant Methods. 2020 Jan 21;16:4. doi: 10.1186/s13007-020-0552-3. eCollection 2020. PubMed Article

Plasmids from Article

ID Plasmid Purpose
135231pBIP1::eYFPUPR signaling reporter (upregulates eYFP expression under UPR conditions)
135232p35S::IRE1aconstitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)
135233p35S::HY5constitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling)
135234p35S::mCh-HA-P2A-lifeact-mChconstitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Antibodies from Article