Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology.
Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C, Fritz-Laylin LK, Buchler NE
Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
135414pEM01Expression of Hygromycin resistance from CMV promoter
135417pEM03Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter
135479pEM09Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP
135482pGI3EM09Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP
135483pGI3EM11Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP
135484pGI3EM18Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato
135487pGI3EM20CAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph
135488pGI3EM22CAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph
135489pGI3EM29Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine
135490pGI3EM30Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3
135491pGI3EM31Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Antibodies from Article