Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology.
Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C, Fritz-Laylin LK, Buchler NE
Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741. PubMed Article
Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C, Fritz-Laylin LK, Buchler NE
Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135414 | pEM01 | Expression of Hygromycin resistance from CMV promoter |
| 135417 | pEM03 | Expression of Hygromycin resistance from Spizellomyces Hsp70 promoter |
| 135479 | pEM09 | Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP |
| 135482 | pGI3EM09 | Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP |
| 135483 | pGI3EM11 | Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP |
| 135484 | pGI3EM18 | Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato |
| 135487 | pGI3EM20C | Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph |
| 135488 | pGI3EM22C | Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph |
| 135489 | pGI3EM29 | Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine |
| 135490 | pGI3EM30 | Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3 |
| 135491 | pGI3EM31 | Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3 |