pEM01
(Plasmid #135414)

• Purpose: Expression of Hygromycin resistance from CMV promoter
• Depositing Lab: Nicolas Buchler
• Publication: Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.

Backbone

• Vector backbone: pRS406
• Backbone size w/o insert (bp): 4287
• Total vector size (bp): 6475
• Modifications to backbone: Insert including CMVpr driving hph (HygR) and ADH1 terminator
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMVpr hph ADH1t
• Alt name: CMV promoter HygR ADH1 terminator
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 2188
• GenBank ID: YP_007349547.1 854068
• Promoter: CMVpr

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CACTTTATGCTTCCGGCTCCTATGTT
• 3′ sequencing primer: CTGCCGGTAGAGGTGTGGTCAATA

Resource Information

• Supplemental Documents:
  • pEM01_sequenced.gbk
• A portion of this plasmid was derived from a plasmid made by: CMVpr from pAB1T7 / hph from pRS306H / ADH1t from pNB780 /

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry