Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells.
Lebar T, Lainscek D, Merljak E, Aupic J, Jerala R
Nat Chem Biol. 2020 Jan 6. pii: 10.1038/s41589-019-0443-y. doi: 10.1038/s41589-019-0443-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135982pcDNA3-His:dCas9:nls:10P3Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells
Loading...
135983pcDNA3-P4:gs:nls:VPRExpresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells
Loading...
135984pcDNA3-P4:gs:nls:FKBPExpresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells
Loading...
135985pcDNA3-Myc:P4:gs:nls:ABIExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells
Loading...
135986pcDNA3-P4:gs:nls:CIBNExpresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells
Loading...
135987pcDNA3-His:FRB:nls:VPRExpresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
Loading...
135988pcDNA3-His:PYL1:nls:VPRExpresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
Loading...
135989pcDNA3-His:CRY2:nls:VPRExpresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
Loading...

Antibodies from Article