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A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells.
Lebar T, Lainscek D, Merljak E, Aupic J, Jerala R
Nat Chem Biol. 2020 Jan 6. pii: 10.1038/s41589-019-0443-y. doi: 10.1038/s41589-019-0443-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135982pcDNA3-His:dCas9:nls:10P3Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells
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135983pcDNA3-P4:gs:nls:VPRExpresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells
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135984pcDNA3-P4:gs:nls:FKBPExpresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells
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135985pcDNA3-Myc:P4:gs:nls:ABIExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells
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135986pcDNA3-P4:gs:nls:CIBNExpresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells
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135987pcDNA3-His:FRB:nls:VPRExpresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
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135988pcDNA3-His:PYL1:nls:VPRExpresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
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135989pcDNA3-His:CRY2:nls:VPRExpresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
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