Fine-tune control of targeted RNAi efficacy by plant artificial small RNAs.
Lopez-Dolz L, Spada M, Daros JA, Carbonell A
Nucleic Acids Res. 2020 May 12. pii: 5836195. doi: 10.1093/nar/gkaa343.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137883 | pENTR-AtTAS1c-D2-B/c | Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+] |
| 137884 | pMDC32B-AtTAS1c-D2-B/c | Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+] |
| 137885 | pMDC32B-AtTAS1c-D2-B/c-AtMIR173 | Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species. |