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Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery.
Velazquez E, Lorenzo V, Al-Ramahi Y
ACS Synth Biol. 2019 Sep 20;8(9):2186-2193. doi: 10.1021/acssynbio.9b00293. Epub 2019 Sep 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138709pSEVA421-Cas9trPlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes
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138711pACDura3Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
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138712pSEVA231-CRISPRPlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array
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