pSEVA231-CRISPR
(Plasmid #138712)

• Purpose: (Empty Backbone) Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array
• Depositing Lab: Víctor de Lorenzo
• Publication: Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-2193. doi: 10.1021/acssynbio.9b00293. Epub 2019 Sep 3.

Backbone

• Vector backbone: pSEVA231
• Backbone manufacturer: Standard European Vector Architecture
• Backbone size (bp): 3123
• Modifications to backbone: The CRISPR array and the leader sequence was excised from pCRISPR (Jiang et al, Nat Biotechnol 2013, 31, 233-239) by restriction with EcoRI and BamHI. The resulting 0.2 Kb fragment was cloned in pSEVA231 restricted with the same enzimes.
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Aparicio T, de Lorenzo V, Martínez-García E (2018) CRISPR/Cas9-based counterselection boosts recombineering efficiency in Pseudomonas putida. Biotechnol J 13(5):1700161
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry