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(Plasmid #138712)


Item Catalog # Description Quantity Price (USD)
Plasmid 138712 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Standard European Vector Architecture
  • Backbone size (bp) 3123
  • Modifications to backbone
    The CRISPR array and the leader sequence was excised from pCRISPR (Jiang et al, Nat Biotechnol 2013, 31, 233-239) by restriction with EcoRI and BamHI. The resulting 0.2 Kb fragment was cloned in pSEVA231 restricted with the same enzimes.
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Aparicio T, de Lorenzo V, Martínez-García E (2018) CRISPR/Cas9-based counterselection boosts recombineering efficiency in Pseudomonas putida. Biotechnol J 13(5):1700161
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA231-CRISPR was a gift from Víctor de Lorenzo (Addgene plasmid # 138712 ; ; RRID:Addgene_138712)
  • For your References section:

    Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery. Velazquez E, Lorenzo V, Al-Ramahi Y. ACS Synth Biol. 2019 Sep 20;8(9):2186-2193. doi: 10.1021/acssynbio.9b00293. Epub 2019 Sep 3. 10.1021/acssynbio.9b00293 PubMed 31419111