Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Multiple Input Sensing and Signal Integration Using a Split Cas12a System.
Kempton HR, Goudy LE, Love KS, Qi LS
Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 10.1016/j.molcel.2020.01.016. PubMed Article

Plasmids from Article

ID Plasmid Purpose
140086pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPREBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance
140087pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPREBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance
140220pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator
140221pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pAConstitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper
140222pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pAConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR
140223pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pAConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator
140224pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pAConstitutive PGK driven expression of intein with miniVPR for 3-way split activator
140226pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance
140227pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPREHypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
140228pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRERRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
140229pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPREDox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP
140230pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPREDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

Antibodies from Article