A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.
Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T
Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. PubMed Article
Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T
Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140601 | pAID1.2-CMV-NmCherry-mAID | Expression of OsTIR1 and mCherry-mAID protein in DT40 cells under the control of CMV promoter |
| 140602 | pAID1.2-CMV-CmCherry-mAID | Expression of OsTIR1 and mAID-mCherry protein in DT40 cells under the control of CMV promoter |
| 140603 | pAID1.2-EF1a-NmCherry-mAID | Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter |
| 140604 | pAID1.2-EF1a-CmCherry-mAID | Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter |
| 140605 | pAID1.2-CMV-NGFP-mAID | Expression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoter |
| 140606 | pAID1.2-CMV-CGFP-mAID | Expression of OsTIR1 and mAID-GFP protein in DT40 cells under the control of CMV promoter |
| 140607 | pAID1.2-EF1a-NGFP-mAID | Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter |
| 140608 | pAID1.2-EF1a-CGFP-mAID | Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter |
| 140609 | pAID-CMV linearizing in pX330 | Crispr/Cas9 plasmid for linearization of pAID-CMV |
| 140610 | pAID-EF1a linearizing in pX330 | Crispr/Cas9 plasmid for linearization of pAID-EF1a |
| 140615 | pAIDFA-EF1a-NmScarlet-mAID | Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter |
| 140616 | pAIDFA-CMV-NmScarlet-mAID | Expression of OsTIR1F74A and mScarlet-mAID protein in DT40 cells under the control of CMV promoter |
| 140617 | pAID1.2-EF1a-NmScarlet-mAID | Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter |
| 140618 | pAID1.2-CMV-NmScarlet-mAID | Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter |