pAID-EF1a linearizing in pX330
(Plasmid
#140610)
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PurposeCrispr/Cas9 plasmid for linearization of pAID-EF1a
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140610 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepX330
- Backbone size w/o insert (bp) 8500
- Total vector size (bp) 8519
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Modifications to backbonesgRNA scaffold has some changes
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA for pAID-EF1a plasmid digestion
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Insert Size (bp)21
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pX330 plasmid was obtained from Addgene (#42230).
Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAID-EF1a linearizing in pX330 was a gift from Tatsuo Fukagawa (Addgene plasmid # 140610 ; http://n2t.net/addgene:140610 ; RRID:Addgene_140610) -
For your References section:
A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T. Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. 10.1093/nar/gkaa748 PubMed 32941625
