pAIDFA-EF1a-NmScarlet-mAID
(Plasmid #140615)

• Purpose: Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter
• Depositing Lab: Tatsuo Fukagawa
• Publication: Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748.

Backbone

• Vector backbone: pBlueScript
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 7346
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsTIR1 mutated F74A
• Species: oriza sativa
• Insert Size (bp): 1725

Resource Information

• Supplemental Documents:
  • pAIDFA-EF1a-NmScarlet-mAID
• A portion of this plasmid was derived from a plasmid made by: mScalet sequence comes from pmScarlet-C1(addgene: #85042)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.01.20.912113v1 for bioRxiv preprint.