pAID-EF1a linearizing in pX330 Citations (3)
Originally described in: A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair.Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, Takahashi K, Torii KU, Fukagawa T Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748. PubMed Journal
Articles Citing pAID-EF1a linearizing in pX330
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| A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines. Nishimura K, Fukagawa T. Bio Protoc. 2021 Jul 20;11(14):e4092. doi: 10.21769/BioProtoc.4092. eCollection 2021 Jul 20. PubMed |
| A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines. Nishimura K, Fukagawa T. Methods Mol Biol. 2022;2377:109-122. doi: 10.1007/978-1-0716-1720-5_6. PubMed |
Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase.
Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y.
Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8.
PubMed
Associated Plasmids |
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