Myosin-X and talin modulate integrin activity at filopodia tips.
Miihkinen M, Gronloh MLB, Popovic A, Vihinen H, Jokitalo E, Goult BT, Ivaska J, Jacquemet G
Cell Rep. 2021 Sep 14;36(11):109716. doi: 10.1016/j.celrep.2021.109716.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 145139 | mScarlet-I-Myo10 ΔF | Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain deletion |
| 145140 | EGFP-FERM (Myo10) | Expresses EGFP-tagged MyTH/FERM domains of MYO10. |
| 145141 | EGFP-MYO10 TF | Expresses EGFP-tagged MYO10 with MyTH/FERM swapped to Talin1 FERM domain. |
| 145142 | pENTR2b ITGB1-tail-mTurquoise2 | Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert |
| 145143 | pENTR2b ITGA2-tail-YPet | Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert |
| 145144 | pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR) | Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation |
| 145816 | EGFP-MYO10 ΔF | Expresses EGFP-tagged MYO10 with MyTH/FERM deletion in mammalian cells |
| 194855 | EGFP-MYO10-FERM-ITGBD | Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells. |
| 194857 | EGFP-MYO10ΔF3 | Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells. |