The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1.
Chang CT, Bercovich N, Loh B, Jonas S, Izaurralde E
Nucleic Acids Res. 2014 Apr;42(8):5217-33. doi: 10.1093/nar/gku129. Epub 2014 Feb 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146809 | pRSFDuetG-HsEDC4_977-1401-HsDCP2_395-420_K | Bacterial Expression of HsEDC4_977-1401-HsDCP2_385-420 |
| 147529 | pCIneo-lambdaN-HA-HsEDC4_974-1401_S | Mammalian Expression of HsEDC4_974-1401 |
| 147790 | pCIneo-lambdaN-HA-HsDCP2_1-244_V | Mammalian Expression of HsDCP2_1-244 |
| 147791 | pCIneo-lambdaN-HA-HsDCP2_1-395_V | Mammalian Expression of HsDCP2_1-395 |
| 147792 | pCIneo-lambdaN-HA-HsDCP2-D15AR19A_V | Mammalian Expression of HsDCP2-D15AR19A |
| 147793 | pCIneo-lambdaN-HA-HsDCP2-F404AF407AF409A_V | Mammalian Expression of HsDCP2-F404AF407AF409A |
| 147794 | pEGFP-N3-HsDCP2_393-420_V | Mammalian Expression of HsDCP2_393-420 |
| 147796 | pSUPERpuro-BglII-HsEDC4-t1_V | Mammalian Expression of HsEDC4-t1-shRNA |
| 147797 | pSUPERpuro-BglII-HsXRN1-t3_V | Mammalian Expression of HsXRN1-t3-shRNA |
| 147799 | pT7-EGFP-C1-HsDCP1a-H19A_V | Mammalian Expression of HsDCP1a-H19A |
| 147800 | pT7-EGFP-C1-HsDCP1a-N74G_V | Mammalian Expression of HsDCP1a-N74G |
| 147801 | pT7-EGFP-C1-HsDCP1a-Q18AH19A_V | Mammalian Expression of HsDCP1a-Q18AH19A |
| 147802 | pT7-EGFP-C1-HsDCP1a-R72G_V | Mammalian Expression of HsDCP1a-R72G |
| 147803 | pT7-EGFP-C1-HsDCP1a-R72GN74G_V | Mammalian Expression of HsDCP1a-R72GN74G |
| 147804 | pT7-EGFP-C1-HsDCP1a-S12AH19A_V | Mammalian Expression of HsDCP1a-S12AH19A |
| 147805 | pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V | Mammalian Expression of HsDCP1a-S12AQ18AH19A |
| 147806 | pT7-EGFP-C1-HsDCP2-5xmut_V | Mammalian Expression of HsDCP2-5xmut |
| 147807 | pT7-EGFP-C1-HsDCP2_1-244_V | Mammalian Expression of HsDCP2_1-244 |
| 147808 | pT7-EGFP-C1-HsDCP2_1-395_V | Mammalian Expression of HsDCP2_1-395 |
| 147809 | pT7-EGFP-C1-HsDCP2_97-392_V | Mammalian Expression of HsDCP2_97-392 |
| 147810 | pT7-EGFP-C1-HsDCP2_97-420_V | Mammalian Expression of HsDCP2_97-420 |
| 147811 | pT7-EGFP-C1-HsDCP2-D15AR19A_V | Mammalian Expression of HsDCP2-D15AR19A |
| 147812 | pT7-EGFP-C1-HsDCP2-E148QF404AF407AF409A_V | Mammalian Expression of HsDCP2-E148QF404AF407AF409A |
| 147813 | pT7-EGFP-C1-HsDCP2-F404AF407AF409A_V | Mammalian Expression of HsDCP2-F404AF407AF409A |
| 147814 | pT7-EGFP-C1-HsDCP2-G96PV97P_V | Mammalian Expression of HsDCP2-G96PV97P |
| 147815 | pT7-EGFP-C1-HsDCP2-K130A_V | Mammalian Expression of HsDCP2-K130A |
| 147816 | pT7-EGFP-C1-HsDCP2-W44A_V | Mammalian Expression of HsDCP2-W44A |