Drosophila Bag-of-marbles directly interacts with the CAF40 subunit of the CCR4-NOT complex to elicit repression of mRNA targets.
Sgromo A, Raisch T, Backhaus C, Keskeny C, Alva V, Weichenrieder O, Izaurralde E
RNA. 2018 Mar;24(3):381-395. doi: 10.1261/rna.064584.117. Epub 2017 Dec 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 147343 | pnEA-NpH-HsNot10_25-707_Q | Bacterial Expression of HsNot10_25-707 |
| 147637 | pnEA-NvM-HsNot7_T | Bacterial Expression of HsNot7 |
| 148287 | pAc5.1B-EGFP-DmBam_AA | Insect Expression of DmBam |
| 148288 | pAc5.1B-EGFP-DmCaf40-dsRNAres_AA | Insect Expression of DmCaf40-dsRNAres |
| 148289 | pAc5.1B-lambdaN-HA-DmBam_AA | Insect Expression of DmBam |
| 148290 | pAc5.1B-lambdaN-HA-DmCaf40-dsRNAres_AA | Insect Expression of DmCaf40-dsRNAres |
| 148731 | pnEA-HsNot10_25-707-HsNot11_257-498-CvH_AF | Bacterial Expression of HsNot10_25-707-HsNot11_257-498 |
| 148732 | pnEA-HsNot11_257-499-CvH_AF | Bacterial Expression of HsNot11_257-499 |
| 148733 | pnEA-NpG-HsNot9_19-285_AF | Bacterial Expression of HsNot9_19-285 |
| 148734 | pnEA-NpH-DmCaf40_25-291_AF | Bacterial Expression of DmCaf40_25-291 |
| 148735 | pnEA-NpH-DmCaf40_25-291-V186E_AF | Bacterial Expression of DmCaf40_25-291-V186E |
| 148736 | pnEA-NpH-DmCaf40_25-291-Y139DG146W_AF | Bacterial Expression of DmCaf40_25-291-Y139DG146W |
| 148737 | pnEA-NpM-HsNot3_1-212_AF | Bacterial Expression of HsNot3_1-212 |
| 148738 | pnEA-NpM-HsNot6_AF | Bacterial Expression of HsNot6 |
| 148739 | pnYC-HsNot1_1-1000_AF | Bacterial Expression of HsNot1_1-1000 |
| 148740 | pnYC-NpM-DmNot1_1468-1719_AF | Bacterial Expression of DmNot1_1468-1719 |
| 148741 | pnYC-NpM-HsNot7_AF | Bacterial Expression of HsNot7 |
| 148742 | pnYC-NvHN-DmBam_13-36_AF | Bacterial Expression of DmBam_13-36 |
| 148815 | pAc5.1B-EGFP-DmBam_1-140_AG | Insect Expression of DmBam_1-140 |
| 148816 | pAc5.1B-EGFP-DmBam_141-422_AG | Insect Expression of DmBam_141-422 |
| 148817 | pAc5.1B-EGFP-DmBam-L17EM24EL28EV32E_AG | Insect Expression of DmBam-L17EM24EL28EV32E |
| 148818 | pAc5.1B-EGFP-DmCaf40-V186E_AG | Insect Expression of DmCaf40-V186E |
| 148819 | pAc5.1B-lambdaN-DmBam-L17EM24EL28EV32E_AG | Insect Expression of DmBam-L17EM24EL28EV32E |
| 148820 | pAc5.1B-lambdaN-HA-DmBam_1-140_AG | Bacterial Expression of DmBam_1-140 |
| 148821 | pAc5.1B-lambdaN-HA-DmBam_141-422_AG | Insect Expression of DmBam_141-422 |
| 148822 | pAc5.1B-lambdaN-HA-DmBam-del13-36_AG | Insect Expression of DmBam-del13-36 |
| 148823 | pAc5.1B-lambdaN-HA-DmBam-L17E_AG | Insect Expression of DmBam-L17E |
| 148824 | pAc5.1B-lambdaN-HA-DmBam-L28E_AG | Insect Expression of DmBam-L28E |
| 148825 | pAc5.1B-lambdaN-HA-DmBam-M24E_AG | Insect Expression of DmBam-M24E |
| 148826 | pAc5.1B-lambdaN-HA-DmBam-V32E_AG | Insect Expression of DmBam-V32E |
| 148827 | pAc5.1B-lambdaN-HA-GST-GGSG-DmBam_13-36_AG | Insect Expression of DmBam_13-36 |
| 148828 | pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG | Mammalian Expression of HsNot9-shRNA2res |
| 148829 | pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG | Mammalian Expression of HsNot9-V181E-shRNA2res |
| 148830 | pnYC-NvM-Bam_13-36_AG | Bacterial Expression of DmBam_13-36 |
| 148831 | pnYC-NvM-DmBam_AG | Bacterial Expression of DmBam |
| 148832 | pnYC-NvM-DmBam_1-140_AG | Bacterial Expression of DmBam_1-140 |
| 148833 | pnYC-NvM-DmBam_141-442_AG | Bacterial Expression of DmBam_141-422 |
| 148834 | pnYC-NvM-DmBam-del13-36_AG | Bacterial Expression of DmBam-del13-36 |
| 148835 | pnYC-NvM-DmBam-L17E_AG | Bacterial Expression of DmBam-L17E |
| 148836 | pnYC-NvM-DmBam-M24E_AG | Bacterial Expression of DmBam-M24E |
| 148837 | pT7-MS2-HA-C1-DmBam_AG | Mammalian Expression of DmBam |
| 148838 | pT7-MS2-HA-C1-DmBam-L17EM24EL28EV32E_AG | Mammalian Expression of DmBam-L17EM24EL28EV32E |
| 148839 | pT7-MS2-HA-C1-MBP-DmBam_13-36_AG | Mammalian Expression of DmBam_13-36 |
| 148840 | pT7-V5-SBP-C1-DmBam_AG | Mammalian Expression of DmBam |
| 148841 | pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG | Mammalian Expression of DmBam_13-36 |