An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.
Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, Weichenrieder O, Izaurralde E
Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837. Epub 2014 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 147787 | pnEA-NpG-CtPAN2opt_1-490_V | Bacterial Expression of CtPAN2opt_1-490 |
| 147788 | pnEA-NpG-NcPAN2opt_18-489_V | Bacterial Expression of NcPAN2opt_18-489 |
| 147789 | pnYC-NpM-CtPAN3opt_171-566_V | Bacterial Expression of CtPAN3opt_171-566 |
| 148002 | pnYC-NpM-NcPAN2_1-321_X | Bacterial Expression of NcPAN2_1-321 |
| 148028 | pAc5.1B-EGFP-DmPAN2_1-358-dsRNAres_Y | Insect Expression of DmPAN2_1-358-dsRNAres |
| 148029 | pAc5.1B-EGFP-DmPAN2_1-468-dsRNAres_Y | Insect Expression of DmPAN2_1-468-dsRNAres |
| 148030 | pAc5.1B-EGFP-DmPAN2_377-1241_Y | Insect Expression of DmPAN2_377-1241 |
| 148031 | pAc5.1B-EGFP-DmPAN2-D1039AE1041A-dsRNAres_Y | Insect Expression of DmPAN2-D1039AE1041A-dsRNAres |
| 148032 | pAc5.1B-EGFP-DmPAN2-del130-133G-dsRNAres_Y | Insect Expression of DmPAN2-del130-133G-dsRNAres |
| 148033 | pAc5.1B-EGFP-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y | Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres |
| 148034 | pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y | Insect Expression of DmPAN2-del246-249-dsRNAres |
| 148035 | pAc5.1B-EGFP-DmPAN2-del358-467-dsRNAres_Y | Insect Expression of DmPAN2-del358-467-dsRNAres |
| 148036 | pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNAres_Y | Insect Expression of DmPAN2-del358-467-T97RH102G-dsRNAres |
| 148037 | pAc5.1B-EGFP-DmPAN2-del468-981-dsRNAres_Y | Bacterial Expression of DmPAN2-del468-971-dsRNAres |
| 148038 | pAc5.1B-EGFP-DmPAN2-del486-493GSG-dsRNAres_Y | Insect Expression of DmPAN2-del486-493GSG-dsRNAres |
| 148039 | pAc5.1B-EGFP-DmPAN2-del975-1031GSSG-dsRNAres_Y | Insect Expression of DmPAN2-del975-1031-GSSG-dsRNAres |
| 148040 | pAc5.1B-EGFP-DmPAN2-del1047-1063GSG-dsRNAres_Y | Insect Expression of DmPAN2-del1047-1063GSG-dsRNAres |
| 148041 | pAc5.1B-EGFP-DmPAN2-dsRNAres_Y | Insect Expression of DmPAN2-dsRNAres |
| 148042 | pAc5.1B-EGFP-DmPAN2-F99EG132EL133E-dsRNAres_Y | Insect Expression of DmPAN2-F99EG132EL133E-dsRNAres |
| 148043 | pAc5.1B-EGFP-DmPAN2-F550EM554EK557E-dsRNAres_Y | Bacterial Expression of DmPAN2-F550EM554EK557E-dsRNAres |
| 148044 | pAc5.1B-EGFP-DmPAN2-F1103A-dsRNAres_Y | Insect Expression of DmPAN2-F1103A-dsRNAres |
| 148045 | pAc5.1B-EGFP-DmPAN2-G132EL133EP134RT137R-dsRNAres_Y | Insect Expression of DmPAN2-G132EL133EP134RT137R-dsRNAres |
| 148046 | pAc5.1B-EGFP-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y | Bacterial Expression of DmPAN2-K1124EY1127EK1131E-dsRNAres |
| 148047 | pAc5.1B-EGFP-DmPAN2-L766EV758E-dsRNAres_Y | Insect Expression of DmPAN3-L766EV758E-dsRNAres |
| 148048 | pAc5.1B-EGFP-DmPAN2-R245E-dsRNAres_Y | Insect Expression of DmPAN2-R245E-dsRNAres |
| 148049 | pAc5.1B-EGFP-DmPAN2-T97RH102G-dsRNAres_Y | Insect Expression of DmPAN2-T97RH102G-dsRNAres |
| 148050 | pAc5.1B-EGFP-DmPAN3-dsRNAres_Y | Insect Expression of DmPAN3-dsRNAres |
| 148051 | pAc5.1B-EGFP-DmPAN3-E679LG683V-dsRNAres_Y | Insect Expression of DmPAN3-E679LG683V-dsRNAres |
| 148052 | pAc5.1B-EGFP-DmPAN3-F658EL622EL665EW707AG711E-dsRNAres_Y | Insect Expression of DmPAN3-F658EL622EL665EW707AG711E-dsRNAres |
| 148053 | pAc5.1B-EGFP-DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres_Y | Insect Expression of DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres |
| 148054 | pAc5.1B-EGFP-DmPAN3-FLLYHRtoEEEAAA-dsRNAres_Y | Insect Expression of DmPAN3-FLLYHRtoEEEAAA-dsRNAres |
| 148055 | pAc5.1B-EGFP-DmPAN3-G711E-dsRNAres_Y | Insect Expression of DmPAN3-G711E-dsRNAres |
| 148056 | pAc5.1B-EGFP-DmPAN3-W707AG711E-dsRNAres_Y | Insect Expression of DmPAN3-W707AG711E-dsRNAres |
| 148057 | pAc5.1B-EGFP-DmPAN3-W707AG711EV765R-dsRNAres_Y | Insect Expression of DmPAN3-W707AG711EV765R-dsRNAres |
| 148059 | pAc5.1B-lambdaN-HA-DmPAN2_1-358-dsRANres_Y | Insect Expression of DmPAN2_1-358-dsRNAres |
| 148060 | pAc5.1B-lambdaN-HA-DmPAN2_1-468-dsRNAres_Y | Insect Expression of DmPAN2_1-468-dsRNAres |
| 148061 | pAc5.1B-lambdaN-HA-DmPAN2_377-1241_Y | Insect Expression of DmPAN2_377-1241 |
| 148062 | pAc5.1B-lambdaN-HA-DmPAN2-D1039AE1041A-dsRNAres_Y | Insect Expression of DmPAN2-D1039AE1041A-dsRNAres |
| 148063 | pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-dsRNAres_Y | Insect Expression of DmPAN2-del130-133G-dsRNAres |
| 148064 | pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y | Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres |
| 148065 | pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y | Insect Expression of DmPAN2-del246-249-dsRNAres |
| 148066 | pAc5.1B-lambdaN-HA-DmPAN2-del358-467-dsRNAres_Y | Insect Expression of DmPAN2-del358-467-dsRNAres |
| 148067 | pAc5.1B-lambdaN-HA-DmPAN2-del468-981-dsRNAres_Y | Insect Expression of DmPAN2-del468-981-dsRNAres |
| 148068 | pAc5.1B-lambdaN-HA-DmPAN2-del486-493GSG-dsRNAres_Y | Insect Expression of DmPAN2-del486-493GSG-dsRNAres |
| 148069 | pAc5.1B-lambdaN-HA-DmPAN2-del975-1031GSSG-dsRNAres_Y | Insect Expression of DmPAN2-del975-1031GSSG-dsRNAres |
| 148070 | pAc5.1B-lambdaN-HA-DmPAN2-del1047-1063-GSG-dsRNAres_Y | Insect Expression of DmPAN2-del1047-1063-GSG-dsRNAres |
| 148071 | pAc5.1B-lambdaN-HA-DmPAN2-dsRNAres_Y | Insect Expression of DmPAN2-dsRNAres |
| 148072 | pAc5.1B-lambdaN-HA-DmPAN2-F99E-dsRNAres_Y | Insect Expression of DmPAN2-F99E-dsRNAres |
| 148073 | pAc5.1B-lambdaN-HA-DmPAN2-F99EG132EL133E-dsRNAres_Y | Insect Expression of DmPAN2-F99EG132EL133E-dsRNAres |
| 148074 | pAc5.1B-lambdaN-HA-DmPAN2-F550EM554EK557E-dsRNAres_Y | Insect Expression of DmPAN2-F550EM554EK557E-dsRNAres |
| 148075 | pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y | Insect Expression of DmPAN2-F1103A-dsRNAres |
| 148076 | pAc5.1B-lambdaN-HA-DmPAN2-G132EL133E-dsRNAres_Y | Insect Expression of DmPAN2-G132EL133E-dsRNAres |
| 148077 | pAc5.1B-lambdaN-HA-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y | Insect Expression of DmPAN2-K1124EY1127EK1131E-dsRNAres |
| 148078 | pAc5.1B-lambdaN-HA-DmPAN2-R245E-dsRNAres_Y | Insect Expression of DmPAN2-R345E-dsRNAres |
| 148079 | pAc5.1B-lambdaN-HA-DmPAN2-T97RH102G-dsRNAres_Y | Insect Expression of DmPAN2-T97RH102G-dsRNAres |
| 148080 | pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y | Insect Expression of DmPAN3-dsRNAres |
| 148081 | pAc5.1B-lambdaN-HA-DmPAN3-E679LG683V-dsRNAres_Y | Insect Expression of DmPAN3-E679LG683V-dsRNAres |
| 148082 | pAc5.1B-lambdaN-HA-DmPAN3-F658EL622EL665EW707AG711E-dsRNAres_Y | Insect Expression of DmPAN3-F658EL622EL665EW707AG711E-dsRNAres |
| 148083 | pAc5.1B-lambdaN-HA-DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres_Y | Insect Expression of DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres |
| 148084 | pAc5.1B-lambdaN-HA-DmPAN3-FLLYHRtoEEEAAA-dsRNAres_Y | Insect Expression of DmPAN3-FLLYHRtoEEEAAA-dsRNAres |
| 148085 | pAc5.1B-lambdaN-HA-DmPAN3-G711E-dsRNAres_Y | Insect Expression of DmPAN3-G711E-dsRNAres |
| 148086 | pAc5.1B-lambdaN-HA-DmPAN3-L766EV768E-dsRNAres_Y | Insect Expression of DmPAN3-L766EV768E-dsRNAres |
| 148087 | pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y | Insect Expression of DmPAN3-W707AG711E-dsRNAres |
| 148088 | pAc5.1B-lambdaN-HA-DmPAN3-W707AG711EV765R-dsRNAres_Y | Insect Expression of DmPAN3-W707AG711EV765R-dsRNAres |
| 148089 | pAc5.1B-lambdaN-HA-DmPAN3-W707E-dsRNAres_Y | Insect Expression of DmPAN3-W707E-dsRNAres |
| 148090 | pnEA-NpH-NcPAN3_234-656_Y | Bacterial Expression of NcPAN2_234-656 |
| 148091 | pnEA-NpH-NcPAN3_234-656-F625E_Y | Bacterial Expression of NcPAN2_234-656-F625E |
| 148092 | pnEA-NpH-NcPAN3_234-656-V627E_Y | Bacterial Expression of NcPAN2_264-656-V627E |
| 148093 | pnEA-NvH-CtPAN3_448-555_Y | Bacterial Expression of CtPAN3_448-555 |
| 148094 | pnYC-NpM-CtPAN2_1-457_Y | Bacterial Expression of CtPAN2_1-457 |
| 148095 | pnYC-NpM-DmPAN2_468-1241_Y | Bacterial Expression of DmPAN2_468-1241 |
| 148096 | pnYC-NpM-NcPan2_1-400_Y | Bacterial Expression of NcPAN2_1-400 |
| 148097 | pnYC-NpM-NcPAN2_1-321-F62E_Y | Bacterial Expression of NcPAN2_1-321-F62E |
| 148098 | pnYC-NpM-NcPAN2_1-321-G97E_Y | Bacterial Expression of NcPAN2_1-321-G97E |
| 148099 | pnYC-NpM-NcPAN2_1-321-P99R_Y | Bacterial Expression of NcPAN2_1-321-P99R |
| 148100 | pnYC-NpM-NcPAN2_1-321-T60R_Y | Bacterial Expression of NcPAN2_1-321-T60R |
| 148101 | pnYC-NpM-NcPAN2_1-351_Y | Bacterial Expression of NcPAN2_1-351 |
| 148102 | pnYC-NpM-NcPAN2_1-368_Y | Bacterial Expression of NcPAN2_1-368 |
| 148103 | pnYC-NpM-NcPAN2_1-390_Y | Bacterial Expression of NcPAN2_1-390 |
| 148104 | pnYC-NpM-NcPAN2_1-461_Y | Bacterial Expression of NcPAN2_1-461 |
| 148105 | pnYC-NpM-NcPAN2_1-1100_Y | Bacterial Expression of NcPAN2_1-1100 |
| 148106 | pnYC-NpM-NcPAN2_342-1100_Y | Bacterial Expression of NcPAN2_342-1100 |
| 148107 | pnYC-NpM-NcPAN2_457-1100_Y | Bacterial Expression of NcPAN2_457-1100 |
| 148108 | pnYC-NpM-NcPAN2_457-1100-D900N_Y | Bacterial Expression of NcPAN2_457-1100-D900N |
| 148109 | pAc5.1B-lambdaN-HA-DmPAN2-G132EL133EP134RT137R-dsRNAres_Y | Insect Expression of DmPAN2-G132EL133E134RT137R-dsRNAres |