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Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm Genetic Requirements in Enterococcus faecalis
Afonina I, Ong J, Chua J, Lu T, Kline KA
mBio. 2020
Article

Plasmids from Article

ID Plasmid Purpose  
153516pMSP3545-dCas9StrExpresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone
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153517pGCP123-EbpA_g1sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded
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153518pGCP123-GFP_g1sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded
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