Imd pathway-specific immune assays reveal NF-kappaB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells.

Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
154152	pKM45	Firefly luciferase with no promoter for negative control for luciferase assays
154153	pKM44	Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter
154154	pKM51	Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site
154155	pKM52	Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site
154156	pKM53	Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel1A NF-κB site
154157	pKM54	Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site
154158	pKM59	Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.
154159	pKM60	Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154160	pKM62	Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154161	pAA1	Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154162	pAA2	Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154163	pAA3	Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
154164	pKM7	mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
154165	pKM28	Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus
154166	pKM5	Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
154167	pKM33	Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
154168	pKM3	Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.