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Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex.
Yang B, Liu H, Liu Z, Doenen R, Nash MA
Nano Lett. 2020 Nov 16. doi: 10.1021/acs.nanolett.0c04178.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
157672pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbRExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.
157673pQE80L-Coh-His-SpyTagExpression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.
157674pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcherExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.
157675pQE80L-His-SpyTag-XMod-Doc(WT)Expression of XMod-Doc wild type with His6x tag and SpyTag.
157676pQE80L-His-SpyTag-XMod(XL2V)-DocExpression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

Antibodies from Article