Overcoming the design, build, test bottleneck for synthesis of nonrepetitive protein-RNA cassettes.

Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein Y, Amit R
Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
158199	CMV-10xPP7 oligo-library based	Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.
158200	CMV-10xMS2 oligo-library based	Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.
158201	CMV-10xQb oligo-library based	Cassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.
158203	CMV-10xPP7-Qb model based	Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.
158204	CMV-10xMS2 no wild type	Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.
158205	CMV-10xQb no wild type	Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.
158206	Ef1a-QCP-3xBFP	Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.