Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo.
Ibraheim R, Tai PWL, Mir A, Javeed N, Wang J, Rodriguez TC, Namkung S, Nelson S, Khokhar ES, Mintzer E, Maitland S, Chen Z, Cao Y, Tsagkaraki E, Wolfe SA, Wang D, Pai AA, Xue W, Gao G, Sontheimer EJ
Nat Commun. 2021 Nov 1;12(1):6267. doi: 10.1038/s41467-021-26518-y. PubMed Article
Ibraheim R, Tai PWL, Mir A, Javeed N, Wang J, Rodriguez TC, Namkung S, Nelson S, Khokhar ES, Mintzer E, Maitland S, Chen Z, Cao Y, Tsagkaraki E, Wolfe SA, Wang D, Pai AA, Xue W, Gao G, Sontheimer EJ
Nat Commun. 2021 Nov 1;12(1):6267. doi: 10.1038/s41467-021-26518-y. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159536 | pEJS1089: mini-AAV.sgRNA.Nme2Cas9 | Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb |
| 159537 | pEJS1099: Dual-sgRNA.Design 4 | Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector |
| 159538 | pEJS1096 Dual-sgRNA.Design 1 | Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector |