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pEJS1099: Dual-sgRNA.Design 4
(Plasmid #159537)


Item Catalog # Description Quantity Price (USD)
Plasmid 159537 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    p1089: minimized AAV.sgRNA.Nme2Cas9
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    Nme2Cas9 with two guide RNA cassettes
  • Alt name
    Neisseria meningitidis Cas9 nuclease
  • Insert Size (bp)
  • Promoter U1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EagI (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer gatctccaccatagcccatc
  • 3′ sequencing primer tgcacaagtatgacctgatcg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEJS1099: Dual-sgRNA.Design 4 was a gift from Erik Sontheimer (Addgene plasmid # 159537 ; ; RRID:Addgene_159537)
  • For your References section:

    Precision Cas9 Genome Editing in vivo with All-in-one, Self-targeting AAV Vectors. Ibraheim R, Tai PWL, Mir A, Javeed N, Wang J, Rodríguez T, Nelson S, Khokhar E, Mintzer E, Maitland S, Cao Y, Tsagkaraki E, Wolfe SA, Wang D, Pai AA, Xue W, Gao G, Sontheimer EJ. bioRxiv October 9, 2020 10.1101/2020.10.09.333997