Detargeting Lentiviral-Mediated CFTR Expression in Airway Basal Cells Using miR-106b.
Choi SH, Reeves RE, Romano Ibarra GS, Lynch TJ, Shahin WS, Feng Z, Gasser GN, Winter MC, Evans TIA, Liu X, Luo M, Zhang Y, Stoltz DA, Devor EJ, Yan Z, Engelhardt JF
Genes (Basel). 2020 Oct 6;11(10). pii: genes11101169. doi: 10.3390/genes11101169.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163918 | pLV-PGK-dt/CBA-EGFP-NLS | Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP. |
| 163919 | pLV-dt/deltaEGFP | PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression. |
| 163921 | pLV-PGK-dt/CBA-EGFP-R106bT | Serves as control to pLV-PGK-dt/CBA-EGFP-106bT. |
| 163923 | pLV-CBA-dt/PGK-CFTR-⌀ | Expresses human CFTR. |
| 163924 | pTripZ-B2 | For making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production. |